What is useful about the HL-60 cell line?
HL-60 is one of the large number of cell lines that could be selected as being highly useful in cancer research. HL-60 cells can be used for assessing chemotherapy drugs and, when differentiated, can be used as a model for vaccine targets.
What are HL-60 cells and why are they interesting in the study of apoptosis?
HL-60 cell model was used to study the effect of DNA topoisomerase (topo) IIα and IIβ on differentiation and apoptosis of cells and is especially useful in dielectrophoresis studies, which require an aqueous environment with suspended and round cells.
How do you subculture a human leukemia cell line HL-60?
Subculture Routine Take a sample of the cell suspension, e.g. 100ul, to count cells. Centrifuge the cell suspension at low speed i.e. 100 – 150 x g for a maximum of 5 mintues. Remove medium and resuspend the cell pellet at a density of 3 – 5 x 100,000 cells/ml in fresh medium containing 20% serum.
Where did the HL-60 cell line come from?
Description: The HL-60 cell line was derived from peripheral blood leukocytes obtained by leukopheresis of a 36-year-old Caucasian female with acute promyelocytic leukemia. It was among the first long-term suspension cultures of human myeloid leukaemic cells to be established.
What’s the best way to culture HL60 cells?
The recommended tips for culturing HL60 are as follows: when starting from a frozen ampoule, add he thawed cells to a conical based t centrifuge tube and add 4ml of culture medium. Take a sample of around 100ul to perform a cell count, and centrifuge the suspension at a low speedto prevent damage to the cells (i.e. 100 – 150 x g for 5 minutes).
How are HL-60 cells differentiated in serum-free cells?
HL-60 cells were cultured in serum (FBS) or serum-free (X-VIVO) medium and differentiated using all-trans retinoic acid (ATRA) or dimethyl sulfoxide (DMSO). dHL-60 cells were stimulated with phorbol 12-myristate 13-acetate (PMA) or Ca2+ionophore (CI). Cell differentiation and apoptosis]
Can a HL-60 cell be used as a neutrophil?
Differentiated human promyelocytic leukemia HL-60 cells (dHL-60) have been used to study the mechanisms of NETs formation, as neutrophils have a short lifespan that limits their use. However, dHL-60 cells are inefficient at generating NETs and therefore are not ideal replacements for neutrophils in studying of NET formation.