What is the purpose of TTC?

What is the purpose of TTC?

The TTC has two major objectives in planning its transit services: to maximize mobility within the City of Toronto; to ensure that transit services are efficient and cost-effective.

What is tetrazolium assay?

Tetrazolium (TZ) testing is a rapid method (can be finished within less than two days) for the evaluation of seed viability. This method has been widely used by seed scientists to assess germination potentials, to determine the extent of seed damage, and to evaluate seed vigor and/or other seed lot problems.

What is the role of 2 3 5 triphenyl tetrazolium chloride in measuring dehydrogenase activity?

The chemical 2,3,5-triphenyltetrazolium chloride was investigated as a possible indicator of cormel dormancy. 2,3,5-triphenyltetrazolium chloride is a water-soluble colorless salt that forms insoluble carmine red triphenyl formazan when in a reduced state.

What is TTC in microbiology?

2,3,5-triphenyltetrazolium chloride (TTC) is a dye largely used for enumeration of microbial colonies in solid culture media, being a key component of the dry rehydratable film system used for microbiological analysis of food.

What is the purpose of tetrazolium chloride?

Triphenyl tetrazolium chloride, TTC, or simply tetrazolium chloride (with the formula 2,3,5-triphenyl-2H-tetrazolium chloride) is a redox indicator commonly used in biochemical experiments especially to indicate cellular respiration (example: to check for the viability of seeds).

What are the three TTC core principles?

More than half a century ago, the deep-seated principles of safety, service and courtesy first appeared in the TTC corporate crest. But with the pressure for modernization and financial efficiency, these core values have not always shone as brightly as they should.

What is TTC full form?

TTC Full Form

How does tetrazolium chloride work?

The amount staining tells the tale. Living tissue contains dehydrogenase enzymes, which reduce the tetrazolium chloride to formazan, a reddish, water-insoluble compound. This reaction only happens in or near living cells that are releasing hydrogen as they respire, so staining indicates the seed is living.

What product of respiration does tetrazolium chloride react with?

Dehydrogenase enzymes present in living tissue reduce the tetrazolium chloride to formazan, a reddish, water-insoluble compound. This reaction occurs in or near living cells, which are releasing hydrogen in respiration processes.

How long is the TTC hiring process?

It took 6 months to get to the actual interview. Applying, skill test, document submission, and than ask to move forward to an interview.

Which is the correct formula for triphenyl tetrazolium chloride?

Triphenyl tetrazolium chloride, TTC, or simply tetrazolium chloride (with the formula 2,3,5-triphenyl-2H-tetrazolium chloride) is a redox indicator commonly used in biochemical experiments especially to indicate cellular respiration (example: to check for the viability of seeds).

How is tetrazolium chloride used in the TTC test?

It is a white crystalline salt, soluble in water, ethanol and acetone but insoluble in ether. In the TTC assay (also known as TTC test or tetrazolium test), TTC is used to differentiate between metabolically active and inactive tissues.

How does the tetrazolium test determine the viability of a seed?

Steps of Tetrazolium test in determining seed viability. Tetrazolium test is done to test the viability of seed. The viability test is done to separate the proportion of living and dead seeds present in the seed sample.

Why is tetrazolium chloride reduced to red TPF?

The white compound is enzymatically reduced to red TPF (1,3,5-triphenylformazan) in living tissues due to the activity of various dehydrogenases (enzymes important in oxidation of organic compounds and thus cellular metabolism), while it remains in its unreacted state in areas of necrosis since these enzymes have…