What is Qubit buffer?

What is Qubit buffer?

buffer. Introduction. The Qubit® dsDNA HS (High Sensitivity) Assay Kits make DNA quantitation easy and accurate. The kits include concentrated assay reagent, dilution buffer, and prediluted DNA standards.

What is Qubit DNA?

Qubit assay dyes bind selectively to DNA, RNA, or protein, making it more specific than UV absorbance. More sensitive than UV absorbance, detecting as little as 10 pg/μl of DNA. Uses as little as 1 μL of sample, even with very dilute samples.

Do qubit reagents expire?

– The kit has expired or has been stored incorrectly: When properly stored, the components of the Qubit Protein Assay Kit should be stable for at least 6 months. Upon receipt, the kit can be stored at 4 degrees C.

Can Qubit measure ssDNA?

Qubit™ ssDNA Assay Kit. New—The Qubit™ 4 Fluorometer can now measure RNA quality in addition to providing accurate quantitation of dsDNA, ssDNA, RNA & protein.

How does the qubit dsDNA HS assay kit work?

Description The Qubit dsDNA HS (High Sensitivity) Assay Kit is designed specifically for use with the Qubit Fluorometer. The assay is highly selective for double-stranded DNA (dsDNA) over RNA and is designed to be accurate for initial sample concentrations from 10 pg/µL to 100 ng/µL.

Which is the best dsDNA assay for fluorometer?

The Qubit dsDNA HS (High Sensitivity) Assay Kit is designed specifically for use with the Qubit Fluorometer. The assay is highly selective for double-stranded DNA (dsDNA) over RNA and is designed to be accurate for initial sample concentrations from 10 pg/µL to 100 ng/µL.

How big of a sample do you need for qubit?

Simply dilute the reagent using the buffer provided, add your sample (any volume between 1 µl and 20 µl is acceptable), and read the concentration using the Qubit Fluorometer. Common contaminants, such as salts, free nucleotides, solvents, detergents, or protein are well tolerated in the assay.

Can a Qubit fluorometer take absorbance readings?

The Qubit fluorometer cannot take absorbance readings to provide a A260/A280 ratio or detect protein in nucleic acid samples. This can be done with the NanoDrop instrument. If your sample contains protein or other contaminants that can affect the assay, it should be further purified.