What is the role of Rho factor in transcription?
Rho factor attaches to the mRNA transcript and uses its helicase function to track along the transcript toward the moving mRNA polymerase [2]. Upon catching up with the polymerase, Rho catalyzes the disassociation of mRNA from genomic DNA and the RNA polymerase [2].
What does Rho subunit do?
the subunit of bacterial RNA Polymerase that appears to be involved in termination of transcription at a Class of sequences known as rho-dependent terminators.
What is Rho in transcription?
The Rho factor is a protein that acts in bacterial cells to mediate termination of transcription at distinct sites. Escherichia coli, and probably most bacteria, have two sets of transcriptional terminators: intrinsic and Rho-dependent.
What is the role of sigma and Rho factor in transcription?
Answer: A sigma factor is a protein needed for initiation of RNA synthesis. It is a bacterial transcription initiation factor that enables specific binding of RNA polymerase to gene promoter. Rho is an ATP-dependent RNA-stimulated helicases that disrupt the nascent RNA-DNA complex.
Is Rho factor an RNA polymerase?
As a molecule, Rho is a RNA/DNA helicase or translocase that dissociates RNA polymerase from DNA template to release RNA, deriving energy by hydrolyzing ATP through its RNA-dependent ATPase activity to bring about termination (Richardson, 2002; Richardson, 2003).
What does rho independent termination of transcription in bacteria involve?
Intrinsic, or rho-independent termination, is a process in prokaryotes to signal the end of transcription and release the newly constructed RNA molecule. A protein bound to RNA polymerase (nusA) binds to the stem-loop structure tightly enough to cause the polymerase to temporarily stall.
What does rho-independent termination of transcription in bacteria involve?
Is Rho factor RNA polymerase?
How does Rho stop transcription?
Rho-dependent termination occurs by binding of Rho to ribosome-free mRNA, C-rich sites being good candidates for binding. Rho’s ATPase is activated by Rho-mRNA binding, and provides the energy for Rho translocation along the mRNA; translocation requires sliding of the message into the central hole of the hexamer.
Which of the following ensures a stable binding of RNA polymerase at promoter site?
Which of the following ensure stable binding of RNA polymerase at the promoter site? Explanation: Sigma factor decreases the binding of the core enzyme to non-specific DNA sequences and increases binding to the promoter.